R4154

Anti-RbAp46, N-terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinônimo(s): Anti-RBBP7, Anti-Retinoblastoma-binding Protein 7
• Número MDL: MFCD08705514
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: IgG fraction of antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: antigen 46 kDa
• reatividade de espécies: human
• técnica(s): indirect immunofluorescence: 1:200-1:400 using human HeLa cells fixed with paraformaldehyde/Triton; western blot: 1:1,000-1:2,000 μg/mL using nuclear extracts of human HeLa cells
• nº de adesão UniProt: Q16576
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... RBBP7(5931)

Descrição

Descrição geral

Retinoblastoma-associated binding protein 46 (RbAp46), also referred to as retinoblastoma-binding protein 7 (RBBP7) has 89% amino acid identity with RbAp48. This nuclear protein belongs to the WD-repeat protein family. It has four repeats that ends with Trp-Asp(WD) residues. RbAp46 is an important constituent of the histone modifying and remodeling complexes. RbAp46 gene is located on human chromosome Xp22.13

Especificidade

Anti-RbAp46, N-terminal specifically recognizes human RbAp46.

Imunogênio

synthetic peptide corresponding to amino acids 1-14 of human RbAp46, conjugated to KLH via a C-terminal added cysteine residue. The sequence differs in mice by one amino acid.

Aplicação

Anti-RbAp46, N-terminal antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Ações bioquímicas/fisiológicas

Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in the modification and remodeling of chromatin during cell growth and differentiation. It is associated with the nucleosomal remodeling and deacetylation complex (NURD). It may also play a key role in histone deacetylation and transcription repression.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)