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P7871

Sigma-Aldrich

Anti-phospho-PAK1/2/3 (pSer141) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

Número MDL:
MFCD06411890
Código UNSPSC:
12352203
NACRES:
NA.44

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous glycerol solution

reatividade de espécies

human

técnica(s)

western blot: 1:1,000 using Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation

nº de adesão UniProt

Q13153

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

phosphorylation (pSer141)

Informações sobre genes

human ... PAK1(5058)

Descrição geral

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of serine 141 (serine 144 for PAK1 and serine 139 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
The antibody detects human PAK1 (46% homologous), PAK2, and PAK3 (46% homologous). Other species have not been tested.

Especificidade

The antibody is highly selective for the phosphorylated form of PAK and can be used to detect an active form of PAK1, as well as PAK2 and PAK3.

Imunogênio

synthetic phosphopeptide derived from the region of human PAK2 that contains serine 141.

Aplicação

A working dilution of 1:1000 is recommended for immunoblotting detection in Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation.

forma física

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Clemens Hofmann et al.
Journal of cell science, 117(Pt 19), 4343-4354 (2004-08-28)
p21-activated kinases (Paks) are a highly conserved family of enzymes that bind to and are activated by small GTPases of the Cdc42 and Rac families. With the notable exception of plants, nearly all eukaryotes encode one or more Pak genes
Mengwei Zang et al.
The Journal of biological chemistry, 277(6), 4395-4405 (2001-12-26)
Activation of Raf-1 is a complex process in which phosphorylation of Ser(338)-Tyr(341) is a critical step. Previous studies have shown that Pak1/2 is implicated in both Ras-dependent and -independent activation of Raf-1 by phosphorylating Raf Ser(338). The present study explores
Zhou-shen Zhao et al.
The Biochemical journal, 386(Pt 2), 201-214 (2004-11-19)
The Rho GTPases are a family of molecular switches that are critical regulators of signal transduction pathways in eukaryotic cells. They are known principally for their role in regulating the cytoskeleton, and do so by recruiting a variety of downstream
C Chong et al.
The Journal of biological chemistry, 276(20), 17347-17353 (2001-03-30)
The p21-activated kinases (PAKs), in common with many kinases, undergo multiple autophosphorylation events upon interaction with appropriate activators. The Cdc42-induced phosphorylation of PAK serves in part to dissociate the kinase from its partners PIX and Nck. Here we investigate in
Christy C Ong et al.
Oncotarget, 2(6), 491-496 (2011-06-10)
The p21-activated kinases (PAKs) are central players in growth factor signaling networks and morphogenetic processes that control proliferation, cell polarity, invasion and actin cytoskeleton organization. This raises the possibility that interfering with PAK activity may produce significant anti-tumor activity. In
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