MAK364
Reduced Glutathione (GSH) Assay Kit (Colorimetric)
sufficient for 100 colorimetric tests
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
uso
sufficient for 100 colorimetric tests
método de detecção
colorimetric
doença(s) relevante(s)
cancer
temperatura de armazenamento
−20°C
Descrição geral
The new Glutathione (GSH) Assay Kit, MAK517, is now available! Glutathione (GSH), a thiol-containing tripeptide (γ-glutamyl-cysteinyl-glycine), is a key antioxidant in many species. It has been highly implicated in the detoxification/elimination of xenobiotics (naturally occurring harmful compounds such as free radicals, hydroperoxides etc.) and in the maintenance of the oxidation state of protein sulfhydryl groups. In addition, GSH plays a pivotal role in the pathogenesis of numerous human diseases including cancer and cardio-vascular diseases. Glutathione is present in cells in both reduced (GSH) and oxidized (GSSG) forms- GSH being, the predominant species under normal physiological conditions inside cells. Thus, pathologic conditions causing oxidative stress would result in increased levels of GSSG. Therefore, the measurement of intracellular GSH appears to be a sensitive indicator of the overall cell health, and its ability of resisting toxic challenges.
Aplicação
<ul>
<li><strong>Hyperbaric oxygen protects HT22 cells and PC12 cells from damage caused by oxygen-glucose deprivation/reperfusion via the inhibition of Nrf2/System Xc-/GPX4 axis-mediated ferroptosis:</strong> This research uses the kit to monitor glutathione levels as part of a strategy to understand cell viability under stress, highlighting its utility in studies involving cellular defense mechanisms against oxidative damage (Chen et al., 2022).</li>
</ul>
<li><strong>Hyperbaric oxygen protects HT22 cells and PC12 cells from damage caused by oxygen-glucose deprivation/reperfusion via the inhibition of Nrf2/System Xc-/GPX4 axis-mediated ferroptosis:</strong> This research uses the kit to monitor glutathione levels as part of a strategy to understand cell viability under stress, highlighting its utility in studies involving cellular defense mechanisms against oxidative damage (Chen et al., 2022).</li>
</ul>
Adequação
The kit is suitable for the measurement of reduced glutathione in various biological samples/preparations including tissue homogenates (liver, etc.) and cell lysates (Hep G2, Jurkat, etc.).
Princípio
The Reduced Glutathione (GSH) Assay Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at A450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample. The kit is highly specific and sensitive because GSSG does not interfere with the assay. The kit includes 5-Sulfosalicylic acid (SSA) in order to protect samples′ GSH endogenous content. (SSA acts as a deproteinizing and antioxidant agent. The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 μL reaction.
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Frases de perigo
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Classificações de perigo
Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
Código de classe de armazenamento
8A - Combustible corrosive hazardous materials
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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