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MAK315

Sigma-Aldrich

Oxalate Assay Kit

Oxalate Assay Kit

sufficient for 100 colorimetric tests

Sinônimo(s):

Oxalic Acid Detection Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

R$ 3.721,00

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método de detecção

colorimetric

doença(s) relevante(s)

genitourinary disorders

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

Oxalate or Oxalic Acid is a metabolic breakdown product of the Kreb′s Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the quantitative determination of oxalate (oxalic acid) in urine, animal and plant tissue samples.

Princípio

Simple, direct and high-throughput assays for measuring oxalate concentration find wide applications. This oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
315CE12A09
315CE12A09
315CE10A18
315CE10A16
315CE10A16

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Natalia Stepanova et al.
The Korean journal of internal medicine (2021-06-26)
It was hypothesized that oxalate might be strongly involved in atherogenesis and the inflammatory pathway that could result in an increased risk of cardiovascular disease (CVD) in end-stage renal disease (ESRD) patients. Therefore, this study aimed to address two primary
Natalia Stepanova et al.
In vivo (Athens, Greece), 36(2), 925-933 (2022-03-05)
Little evidence is available on oxalate balance in peritoneal dialysis (PD) patients. We performed a cross-sectional observational pilot study with 62 adult PD patients to document oxalate balance and explore its association with PD-related peritonitis. Plasma oxalate concentration, levels of
Natalia Stepanova et al.
Urolithiasis, 50(3), 249-258 (2022-02-08)
The present study aimed (i) to evaluate whether ceftriaxone treatment could affect not only intestinal oxalate-degrading bacteria number but also their total activity to degrade oxalate and influence oxalate homeostasis in rats, (ii) and to estimate the ability of commercially
Anna Kletzmayr et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(8), 1903337-1903337 (2020-04-25)
Calcium oxalate (CaOx) crystal-induced nephropathies comprise a range of kidney disorders, for which there are no efficient pharmacological treatments. Although CaOx crystallization inhibitors have been suggested as a therapeutic modality already decades ago, limited progress has been made in the
1 Review This action will navigate to reviews.

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the purpose of acidifying the samples for longer-term storage?

    1 answer

    1. Acidification of urine samples is necessary for longer-term storage. Oxalate demonstrates greater solubility under acidic conditions. Additionally, ascorbic acid (Vitamin C) in urine samples rapidly degrades into oxalate in non-acidified samples, so acidifying urine should help to prevent this. Given that the Working Reagent used in this assay is acidic, acidifying fresh samples prior to the assay should not be necessary.

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  4. What is the approximate expiration time for these kits?

    1 answer

    1. Expiration dating is batch specific and should be determined from the lot specific Certificate of Analysis. Please review your batch specific Certificate of Analysis by searching your lot number in the DOCUMENTATION section of the Product Detail Page:
      https://www.sigmaaldrich.com/product/sigma/MAK315#product-documentation

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Reviews

Active Filters

  1. Nashua, NH
    • Review 1
    • Votes 0
    1 out of 5 stars.

    Oxalate Assay Kit has Issues

    Poor instructions with kit. Poor precision of assay with urine samples.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for reaching out and sharing your review with us. We are sorry to hear about your experience with this product and is not what we would expect. The Oxalate Assay Kit does include a three page protocol with calculation and troubleshooting sections, but we would love to get more of your feedback on how to improve the instructions. Variables such as sample age and preservation can impact results. A positive control in included to ensure valid results. When you have a moment, please call or email us for additional technical support by visiting https://www.sigmaaldrich.com/support/customer-support and submit a Product Technical Inquiry. We hope to hear from you soon.

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