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MAK190

Sigma-Aldrich

ATP Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Sinônimo(s):

Adenosine triphosphate Assay Kit

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About This Item

Número CE:
213-579-1
Código UNSPSC:
12161503
NACRES:
NA.84

método de detecção

colorimetric
fluorometric

doença(s) relevante(s)

cancer; neurological disorders

temperatura de armazenamento

−20°C

Descrição geral

Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes.[1][2] Genetic disorders such as Leber hereditary optic neuropathy,[2] Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa [3] affect the generation of ATP in the mitochondria.

Aplicação

ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.[4]

Adequação

Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.

Princípio

The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.

Outras notas

The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.

Somente componentes do kit

Nº do produto
Descrição
  • ATP Assay Buffer
  • ATP Probe, in DMSO
  • ATP Converter
  • Developer Mix
  • ATP Standard, 1.0 μmole

substituído por

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

188.6 °F

Ponto de fulgor (°C)

87 °C

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Certificados de análise (COA)

Lot/Batch Number
8L09K03540
8E04K03540
8A07K03540
7H23K03540
7D05K03540

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Pesquise por COA

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Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the chemical composition of the reagents?

    1 answer

    1. The chemical composition of these reagents is proprietary.

      Helpful?

  4. Dear sir, We are interested in quantification of ATP levels "ATP Assay Kit" Cat.No:MAK190. Protocol recommends that cells should be deproteinized using 10kDa MWCO spin filter, but no protocol is mentioned. Can you Please send us protocol for the same.

    1 answer

    1. The Datasheet can be accessed in the DOCUMENTATION section under 'More Documents'. Please see the Datasheet below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

      Helpful?

  5. What is the chemical composition of the reagents?

    1 answer

    1. The chemical composition of these reagents are proprietary. Please see the link below to review the Product datasheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

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