immunocytochemistry: 1:10-1:20 using 4% paraformaldehyde-fixed cytospin preparations immunohistochemistry (frozen sections): 1:10-1:20 western blot (chemiluminescent): 1:10-1:20
Minor vault protein 193 is also known as poly (ADP-ribose) polymerase family member 4 (PARP4). Along with two other proteins, it is part of vaults (ribonucleoprotein particles present in the cytoplasm). The molecular weight of this protein is 193kDa.
Especificidade
Reacts with an internal epitope (aa 506-510, VALGK) of p193.
Imunogênio
E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.
Ações bioquímicas/fisiológicas
Minor vault protein 193 is highly expressed in tumors.
forma física
Supplied in serum-free culture medium containing 0.7% bovine serum albulin and 0.1% sodium azide.
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Expression profiles of vault components MVP, TEP1 and vPARP and their correlation to other multidrug resistance proteins in ovarian cancer.
Szaflarski W
International Journal of Oncology, 43(2), 513-520 (2013)
The 193-kD vault protein, VPARP, is a novel poly(ADP-ribose) polymerase.
Kickhoefer VA
The Journal of Cell Biology, 146(5), 917-928 (1999)
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