M6320

Monoclonal Anti-MAGE-1 antibody produced in mouse

~2 mg/mL, clone MA454, purified immunoglobulin, buffered aqueous solution

• Sinônimo(s): Anti-Melanoma-associatiated antigen 1
• Número MDL: MFCD03096998
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: MA454, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~46 kDa
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• concentração: ~2 mg/mL
• técnica(s): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 5-10 μg/mL using total cell extract of SKML37 cells
• Isotipo: IgG1
• nº de adesão UniProt: P43355
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MAGEA1(4100)

Descrição

Descrição geral

Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen.[1][2] MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28.[3] Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.

Especificidade

The antibody does not recognize MAGE-2 and MAGE-3.[4][5]

Imunogênio

recombinant human MAGE-1.

Aplicação

Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:

• ELISA
• immunoblotting
• immunohistochemistry

Ações bioquímicas/fisiológicas

The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC).[6] Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)