H6412

Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse

2.0-2.5 mg/mL, clone HDAC8-48, purified immunoglobulin, buffered aqueous solution

• Número MDL: MFCD04975119
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: HDAC8-48, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~43 kDa
• reatividade de espécies: human
• concentração: 2.0-2.5 mg/mL
• técnica(s): indirect ELISA: suitable; microarray: suitable; western blot: 4 μg/mL using nuclear extracts of HeLa cells
• Isotipo: IgG1
• nº de adesão UniProt: Q9BY41
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HDAC8(55869)

Descrição

Descrição geral

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2, HDAC3, and HDAC8 belong to Class I HDACs and HDAC4, HDAC6, HDAC7, HDAC9, and HDAC10 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. Studies show that HDAC8 is a sex-linked gene located on the chromosome at position Xq21.2 - q21.3. HDAC8 gene has a molecular weight of 43kDa and it encodes a 377 amino acid protein. It is present within the nucleas. HDAC8 mRNA is seen in heart, lung, kidney, and pancreas.

Monoclonal Anti-Histone Deacetylase 8 (HDAC8) (mouse IgG1 isotype) is derived from the HDAC8-48 hybridoma produced by the fusion of NS-1 mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human HDAC8.

Imunogênio

recombinant human HDAC8

Aplicação

Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse has been used in :

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunofluorescence staining

Ações bioquímicas/fisiológicas

Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing. HDAC8 controls HDAC activity on H4 histone peptide substrates. HDAC8 is similar to the HDAC class I enzymes. In vitro expression and activity of HDAC8 was examined using FLAG tagged- HDAC8 and HDAC1. These were transfected into HeLa cells and Sf9 insect cells. After the immunoprecipitation of cell lysates the expression results were confirmend using Western blotting. Studies show that HDAC8 may play a role in transcriptional regulation and could possibly be regulated in a temporal or compartment-specific manner.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.