The lyophilized powder may be reconstituted at 5 mg/mL in 0.02M sodium phoshate buffer or 0.45% sodium chloride containing 0.01% BSA. Solutions prepared at concentrations of at least 1 mg/ml may be stored in aliquots at -20°C for several months.
H2126
Hyaluronidase from sheep testes
Type II, lyophilized powder, ≥300 units/mg solid
Sinônimo(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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100 MG
R$ 596,00
500 MG
R$ 1.621,00
1 G
R$ 2.749,00
5 G
R$ 11.742,00
R$ 596,00
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100 MG
R$ 596,00
500 MG
R$ 1.621,00
1 G
R$ 2.749,00
5 G
R$ 11.742,00
About This Item
Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
R$ 596,00
Check Cart for Availability
conjugado
conjugate (Glucosaminoglycan)
tipo
Type II
Formulário
lyophilized powder
atividade específica
≥300 units/mg solid
peso molecular
55 kDa
temperatura de armazenamento
−20°C
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Descrição geral
Hyaluronidase is a hyaluronic acid-metabolizing enzyme.[1] It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.[2]
Aplicação
Hyaluronidase from sheep testes has been used:
- in flow cytometry[3]
- in the isolation of putative spermatogonial stem cells (SSCs)[4]
- to study the effects of magnetic stimulation in magnetic hydrogel properties[5]
- in immunohistochemistry to visualize collagen type II distribution[6][7]
- to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings[8]
- for the degeneration of hydrogels[9]
Ações bioquímicas/fisiológicas
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Definição da unidade
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
forma física
Lyophilized powder containing lactose
Outras notas
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Já possui este produto?
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Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface
Silva ED, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine, 14(7), 2375-2385 (2018)
Side Effects of Drugs Annual: A world-wide yearly survey of new data and trends in adverse drug reactions., 603-619 (2010)
Suppression of Sertoli cell tumour development during the first wave of spermatogenesis in inhibin $\alpha$-deficient mice
Haverfield JT, et al.
Reproduction, Fertility, and Development, 29(3), 609-620 (2017)
Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis
Binsila KB, et al.
Animal Reproduction Science, 609-620 (2018)
The role of the mechanotransduction ion channel candidate Nanchung-Inactive in auditory transduction in an insect ear.
Warren B and Matheson T
The Journal of Neuroscience, 2310-2317 (2018)
Artigos
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Protocolos
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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Kann die Hyaluronidase H2126 in PBS gelöst werden um sie dann in kleinen Aliquoten bei -20 C zu lagern?
1 answer-
Helpful?
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H2126-100MG Hyaluronidase from sheep testes についてですが、溶解方法を教えてください。
1 answer-
This enzyme is soluble at 1 mg/mL in 20 mM sodium phosphate buffer (pH 7.0), with 77 mM sodium chloride and 0.1 mg/mL BSA at 1 mg/mL. For assay purposes, this product is prepared at 0.3 mg/mL solution in Phosphate Buffer. Heating may be required, Please see the link below to review the full assay protocol:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase#preparationPlease see the link below to review the product datasheet for a bovine Hyaluronidase, product H3884. This information may also be applied to the ovine source material:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdfHelpful?
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