The low value observed in the sample may be due to several factors. Since the standard curve is working well, it is likely that the lysis buffer and/or homogenization condition used is not optimized for assay reading. To verify the possible presence of inhibiting substances, one could add a known amount of ATP from a standard solution to the homogenate to see if the reading increases accordingly. It's possible that the lysis buffer used was not optimal to yield an adequate amount of ATP for detection. Currently, there is no recommended cardiac tissue preparation protocol for use with this kit. As the kit measures only soluble ATP, it is highly recommended to clarify the homogenized extract by centrifugation to obtain a supernatant solution for use with this assay.
FLAAB
Adenosine 5′-triphosphate (ATP) assay mix dilution buffer
lyophilized powder
Sinônimo(s):
ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP
Selecione um tamanho
| Tamanho/SKU | Disponibilidade | Preço |
|---|
Sobre este item
form
lyophilized powder
Quality Segment
technique(s)
activity assay: suitable, protein detection: suitable
storage temp.
−20°C
General description
Application
Preparation Note
1 of 1
Este Item | |||
|---|---|---|---|
| description lyophilized powder | description lyophilized powder | description for ATP quantitation | description vial of ~1 mg ATP |
| technique(s) activity assay: suitable | technique(s) PCR: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form - | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Palavra de aviso
Danger
Códigos de risco
Declarações de precaução
Hazard Classifications
Eye Dam. 1
Classe de armazenamento
11 - Combustible Solids
O que está acontecendo?
WGK 3
Ponto de inflamação (°F)
Not applicable
Ponto de inflamação (°C)
Not applicable
EPI (equipamento de proteção individual)
Eyeshields, Gloves, type N95 (US)
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
-
It seems that you are currently trying to perform the ATP assay (Sigma Cat# FLAA) on cardiac tissue. The kit appears to be working, as your standard curve is functioning, but you are obtaining very low numbers for your samples, significantly below your standard curve. Given that the heart should contain an abundance of ATP, you are concerned that your extraction protocol might be the issue. You are wondering if there is an extraction protocol available for this tissue for the mentioned kit.
1 answer-
Helpful?
-