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Key Documents

F5303

Sigma-Aldrich

Monoclonal Anti-Falkor/PHD1 antibody produced in mouse

clone FLK-49, purified immunoglobulin, buffered aqueous solution

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

FLK-49, monoclonal

forma

buffered aqueous solution

reatividade de espécies

mouse

técnica(s)

microarray: suitable
western blot: 0.5 μg/mL using total cell extracts from 293T cells transfected with Falkor-myc tagged

Isotipo

IgG2a

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

mouse ... Egln2(112406)

Descrição geral

Falkor is expressed at different levels in various tissues. It is localized in the nuclei of cells and encoded by 2Kb mRNA. Falkor is ubiquitously expressed in various mouse tissue with high level expression in testis and the lowest expression in spleen and skeletal muscle.
Monoclonal Anti-Falkor/PHD1 (mouse IgG2a isotype) is derived from the FLK-49 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The PHD1 gene is mapped to human chromosome 19q13.2and exists in three isoforms.

Especificidade

Monoclonal Anti-Falkor/PHD1 recognizes mouse Falkor/PHD1 (approx. 47 kDa).

Imunogênio

synthetic peptide corresponding to amino acids 2-20 of falkor/PHD1.

Aplicação

Monoclonal Anti-Falkor/PHD1 antibody is suitable for microarray and western blot at a concentration of 0.5μg/mL using total cell extracts from 293T cells transfected with Falkor-myc tagged.
Monoclonal Anti-Falkor/PHD1 antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Ações bioquímicas/fisiológicas

EGLN2 (Egl nine homolog 2), also known as prolyl hydroxylase domain-containing protein 1 (PHD1), functions as an oxygen sensor in cells and mediates oxygen homeostasis. It inhibits nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB) signaling. Polymorphisms in the EGLN2 is implicated in various colorectal, hepatocellular and non-small cell lung cancer.
Falkor, a novel cell growth regulator, is speculated that the falkor protein is associated with suppression of cell growth and comprises of several putative phosphorylation sites for proteins such as protein kinase C and casein kinase II. Falkor has a significant homology to the C.elegans EGL-9. The human homolog of Falkor and EGL-9 is PHD1 (prolyl hydroxylase 1) which is involved in the regulation of HIF-1α. PHD1 function as dioxygenases that regulate HIF-1α by prolyl hydroxylation.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hamid Bur et al.
Anticancer research, 38(1), 329-336 (2017-12-27)
Hypoxia-inducible factors (HIFs) and prolyl hydroxylase domain (PHD) proteins control cellular oxygen homeostasis and a wide range of other processes. We immunohistochemically assessed the expression of HIF1α, HIF2α, PHD1, PHD2 and PHD3 in 115 cases of classical Hodgkin's lymphoma, all
Mohammad Hashemi et al.
Asian Pacific journal of cancer prevention : APJCP, 19(4), 923-926 (2018-04-26)
It has been shown that a 4-bp insertion/deletion (ins/del) polymorphism of EGLN2 influences the risk of several cancers. However, to date, no study has inspected the impact of the 4-bp ins/del polymorphism on breast cancer (BC) risk. A case-control study
Jianhe Huang et al.
The Journal of biological chemistry, 277(42), 39792-39800 (2002-08-16)
Hypoxia-inducible factor (HIF) is a heterodimeric transcription factor induced by hypoxia. Under normoxic conditions, site-specific proline hydroxylation of the alpha subunits of HIF allows recognition by the von Hippel-Lindau tumor suppressor protein (VHL), a component of an E3 ubiquitin ligase
Dorothy M Ryan et al.
PloS one, 9(2), e88051-e88051 (2014-02-06)
Genome-wide association studies (GWAS) and candidate gene studies have identified a number of risk loci associated with the smoking-related disease COPD, a disorder that originates in the airway epithelium. Since airway basal cell (BC) stem/progenitor cells exhibit the earliest abnormalities
Neta Erez et al.
Oncogene, 21(44), 6713-6721 (2002-10-03)
A novel cell growth regulator, named Falkor, was identified using a functional approach to mammalian gene cloning, the Genetic Supressor Elements (GSE) method. In this screen, expression of the C-terminal domain of Falkor conferred cells with resistance to cisplatin-induced growth

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