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EPI001

Sigma-Aldrich

Histone Acetyltransferase (HAT) Activity Assay Kit

100 assays in 96 well plates

Sinônimo(s):

HAT Activity, Histone Acetyltransferase Activity

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About This Item

Número da licença da enzima:
2.3.1.48 (BRENDA, IUBMB)
Código UNSPSC:
12352200
NACRES:
NA.41

uso

100 assays in 96 well plates

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

Histone acetyltransferases (HATs) have critical roles in cellular functions and are involved in the regulation of gene transcription, differentiation, and proliferation. The HAT Activity Colorimetric Assay Kit offers a convenient, non-radioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit includes an active Nuclear Extract (NE) to be used as a positive control, HAT cofactor, acetyl-CoA, and all downstream cofactors and substrates. Acetylation of a peptide substrate by active HAT releases the free form of CoA, which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous, and therefore, is suitable for kinetic studies. The kit provides all reagents and a simple, straightforward protocol for a complete assay.

Características e benefícios

  • Sensitive and reliable assay
  • Utilizes colorimetric methods
  • Sample type: cell and tissue lysates
  • Species reactivity: mammalian
  • Suitable for individual tests or high throughput assays and kinetic studies
  • Easy and simple procedure: combine nuclear extract or protein sample with reagents, incubate, and read absorbance
  • Convenient 96-well microplate format
  • Continuously measures HAT activity; suitable for kinetic studies
  • No HDAC interference; crude nuclear extract can be used directly

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Certificados de análise (COA)

Lot/Batch Number
4M07K03320
B2G120332V

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