E2129

Evans Blue

Dye content ≥75 %, Powder

• Sinônimo(s): Direct Blue 53
• Fórmula empírica (Notação de Hill): C₃₄H₂₄N₆Na₄O₁₄S₄
• Número CAS: 314-13-6
• Peso molecular: 960.81
• Número do índice de cores: 23860
• Número CE: 206-242-5
• Número MDL: MFCD00004021
• Código UNSPSC: 12171500
• ID de substância PubChem: 24894456
• NACRES: NA.47

Propriedades

• product name: Evans Blue, Dye content ≥75 %
• forma: powder
• Nível de qualidade: 200
• composição: Dye content, ≥75%
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
• chave InChI: ATNOAWAQFYGAOY-GPTZEZBUSA-J
• Informações sobre genes: human ... PTPN1(5770) , PTPRF(5792)

Descrição

Descrição geral

Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.

Aplicação

Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.

Ações bioquímicas/fisiológicas

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H350
• Declarações de precaução: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Carc. 1B
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable