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Sigma-Aldrich

Trypan Blue

≥80% (HPLC), Dye content 60 %

Sinônimo(s):

Direct blue 14

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About This Item

Fórmula linear:
C34H24N6O14S4Na4
Número CAS:
Peso molecular:
960.81
Número do índice de cores:
23850
Beilstein:
4360496
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47

Ensaio

≥80% (HPLC)

forma

powder

composição

Dye content, 60%

cor

dark green to brown, or Brown-Black

pf

>300 °C (lit.)

solubilidade

H2O: 1 mg/mL

λmax

607 nm

ε (coeficiente de extinção)

≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

chave InChI

GLNADSQYFUSGOU-UHFFFAOYSA-J

Informações sobre genes

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Descrição geral

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.

Aplicação

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Ações bioquímicas/fisiológicas

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to visualize cell death.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Carc. 1B

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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