CLLS1096
DLD1 Cells SMAD4 -/-
human male colon (Source Disease: Colorectal adenocarcinoma)
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About This Item
Código UNSPSC:
41106514
NACRES:
NA.81
Preço e disponibilidade não estão disponíveis no momento.
Nome do produto
DLD1 Cells SMAD4 -/-,
fonte biológica
human male colon (Source Disease: Colorectal adenocarcinoma)
nº de adesão OMIM
temperatura de armazenamento
−196°C
Informações sobre genes
human ... SMAD4(4089)
Descrição geral
DLD1 Cells SMAD4 -/- are colorectal adenocarinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.
This product corresponds to ATCC Cat. No. CCL-221.
Aplicação
Media Renenewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:3 to 1:10.
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:3 to 1:10.
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Características e benefícios
These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 18q21.1
Zinc finger nuclease (ZFN) knock out on chromosome 18q21.1
Qualidade
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Exoneração de responsabilidade
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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