Lu2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Mouse x Mouse Hybridoma
• embalagem: tube of 5 μg 99043003-DNA-5UG; pkg of vial of cells 99043003-1VL
• ImmunoDon: BALB/c x C57 Bl/6
• Mieloma: P3x63Ag8.653
• Reatividade: Anti-DR11
• técnica(s): cell culture | mammalian: suitable
• Isotipo: IgM
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse x Mouse hybridoma

Descrição de linhagem celular

The cell line Lu2 has been established by fusion of the myeloma P3x63Ag8.653 and spleen cells from BALB/c x C57Bl/6 female mice. Mice received a single i.p. injection with the B cell line LuMar expressing HLA A2.3/B18.5/C5/DR3,11.and B. pertussis three days before fusion. The cell line has been eradicated from mycoplasma using Cibrobay.

Imunogênio

B cell line LuMar

Aplicação

Immunofluorescence, cytotoxicity, immunoprecipitation

Meio de cultura

RPMI 1640 + 2mM glutamine + 10% FBS

Rotina de subcultura

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.