LB198

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human blood
• embalagem: tube of 5 μg 98062320-DNA-5UG; pkg of vial of cells 98062320-1VL
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human lymphocytes, Trichothiodystrophy heterozygote, EBV-transformed

Descrição de linhagem celular

LB198 has been derived from mononuclear cells from peripheral blood of a 45 year old female. The cells have been transformed with EBV. The patient has been shown to be heterozygote for trichothiodystrophy (TTD) and is the parent of LB199 (Sigma Catalogue number.98062321) TTD is a rare genetic disorder with deficiency in excision repair of UV damage. Fibroblasts from the same individual have been designated TTD HM 1BR.

Aplicação

DNA repair deficiency studies

Meio de cultura

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Rotina de subcultura

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Outras notas

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