SK-N-BE(2)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95011815, human nerve, Epitheilioid & neuroblastoid

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human nerve
• embalagem: tube of 5 μg 95011815-DNA-5UG; pkg of vial of cells 95011815-1VL
• modo de crescimento: Adherent
• cariótipo: Modal no. 44
• morfologia: Epitheilioid & neuroblastoid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian neuroblastoma

Descrição de linhagem celular

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

Aplicação

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

Meio de cultura

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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