One of a series of cell lines established from mononuclear cells of a boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). The cells contain retrovirus particles and are positive in RT assay.
Perfil do DNA
Not specified
Meio de cultura
RPMI 1640 + 2 mM Glutamine + 1 mM Sodium Pyruvate (NaP) + 0.05 mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS).
Rotina de subcultura
Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37 °C. Cells grow as small clumps.
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