BLO-11

90112702, mouse muscle, Fibroblast-like

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: BLO-11, 90112702
• fonte biológica: mouse muscle
• modo de crescimento: Adherent
• cariótipo: 2n = 40
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Moblo/y muscle hereditary emphysema

Descrição de linhagem celular

Established from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.

Aplicação

Genetic studies

Meio de cultura

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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