M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: M-MSV-BALB/3T3, 90030802
• fonte biológica: mouse embryo
• modo de crescimento: Adherent
• cariótipo: Modal no. 57
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

Descrição de linhagem celular

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

Aplicação

Transformation and virus studies: M-MSV, MuLV

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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