HL60 induced to mature into neutrophils with 1.75% DMSO after treating with SV40. Fails to express SV40 specific T Ag suggesting that HL60 M4 represents expansion of sub-clones of HL60 with inherent restricted capacity for neutrophil differentiation.
Maintain cultures between 3-9x100,000 cells/ml. Do not allow the culture medium to become acidic or the cells will differentiate; 5% CO2; 37°C.
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