THM 7.3.5

Reactivity: Anti-NP (4-hydroxy-3 nitrophenacetyl), 87120201

• Código UNSPSC: 12352200

Propriedades

• fonte biológica: Mouse + Human Transfectoma
• ImmunoDon: Not applicable
• Mieloma: J558L
• Reatividade: Anti-NP (4-hydroxy-3 nitrophenacetyl)
• técnica(s): cell culture | mammalian: suitable
• Isotipo: IgM
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse J558L + Mouse VH gene + Human C(μ) gene

Descrição de linhagem celular

This human chimeric antibody is specifically directed against the hapten-4-hydroxy-3-nitrophenacetyl. V lambda of the endogenous light chain of plasmacytoma line complements VNP of transfected heavy chain to yield specific antibody. Gene marker: gpt.

Meio de cultura

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Outras notas

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.