Ehrlich-Lettre Ascites strain E cell line

87032503, mouse unknown/unspecified, Epithelial

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: Ehrlich-Lettre Ascites strain E cell line, from mouse unknown/unspecified, 87032503
• fonte biológica: mouse unknown/unspecified
• modo de crescimento: Adherent
• cariótipo: 2n = 40
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse carcinoma

Descrição de linhagem celular

Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.

Aplicação

Anti-tumour test and tumourigenicity studies

Meio de cultura

NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change 3 times a week.

Outras notas

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