LIM2551
12070505, human colon, epithelial; forms aggregates of small rounded cells, adherent and suspended.
Sinônimo(s):
LIM 2551, LIM-2551
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About This Item
Código UNSPSC:
41106514
Produtos recomendados
product name
LIM2551, 12070505
fonte biológica
human colon
modo de crescimento
Semi-adherent
cariótipo
Not specified
morfologia
Epithelial; grows as loose aggregates of small rounded cells, some adherent, others in suspension.
receptores
Not specified
doença(s) relevante(s)
cancer
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Origem de linhagem celular
Human derived from an adenocarcinoma of the transverse colon
Descrição de linhagem celular
LIM2551 was derived from an adenocarcinoma of the transverse colon. Cell Bank Australia reference number: CBA0170.
Perfil do DNA
Not specified
Meio de cultura
RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442), Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM.
Rotina de subcultura
Optimal split ratio 1:8-1:16 (seeding density 1 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. LIM2551 requires the growth medium to be changed 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).
Outras notas
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia
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