LIM2099 cell line human

12062002, human liver (metastasis), epithelial

• Sinônimo(s): LIM 2099, LIM-2099
• Código UNSPSC: 41106514

Propriedades

• product name: LIM2099 cell line human, 12062002
• fonte biológica: human liver (metastasis)
• modo de crescimento: adherent
• morfologia: epithelial
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição de linhagem celular

Human liver metastasis, moderately differentiated sclerosing adenocarcinoma. Cell Bank Australia reference number: CBA0164.

Meio de cultura

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10μM

Rotina de subcultura

Optimal split ratio 1:4-1:6 (seeding density 0.8 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5-10 min.

Outras notas

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