NS20Y-BU-4

8062503, mouse nerve, Neuronal

• Código UNSPSC: 41106514

Propriedades

• product name: NS20Y-BU-4, 08062503
• fonte biológica: mouse nerve
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Neuronal
• produtos: Choline acetyltransferase
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse neuroblastoma

Descrição de linhagem celular

A bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.

Aplicação

Used in the generation of cell hybrids.

Meio de cultura

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridine

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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