PNV35B

01080924, Reactivity: Anti-Osteoclasts (human foetal), ECV304 and U937 cells

• Código UNSPSC: 41106514

Propriedades

• ImmunoDon: BALB/c mice
• Mieloma: Ag876
• Reatividade: Anti-Osteoclasts (human foetal), ECV304 and U937 cells
• técnica(s): cell culture | mammalian: suitable
• Isotipo: IgM plus IgG
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Anti-Osteoclastoma Hybridoma

Descrição de linhagem celular

135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.

Imunogênio

Osteoclastoma tumour

Aplicação

Immunocytochemistry

Meio de cultura

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)

Rotina de subcultura

Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO₂, 37°C,

Outras notas

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