C-12907

Human Mononuclear Cells (hMNC)

Isolated from peripheral blood, single donor, ultra-pure, 25 million cryopreserved cells

• Sinônimo(s): hMNC cells
• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• fonte biológica: human peripheral blood
• embalagem: pkg of 25,000,000 cells
• morfologia: (mononuclear)
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

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Origem de linhagem celular

Blood

Aplicação

Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

Qualidade

Rigid quality control tests are performed for each lot of Mononuclear Cells. They are routinely characterized by flow cytometry analyzing a series of cellular parameters, e.g. viability, cell size, granularity. provides detailed information on the percentages of the major cellular sub-populations, i.e., the lymphocytes, the monocytes, and granulocytes, for each lot of Mononuclear Cells. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertência

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rotina de subcultura

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Outras notas

Recommended Plating Density: 1 x 106 cells per mlTested Markers: CD31 negative, CD34 negative, CD105 positive, CD146 positive

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable