B9673

1-Bromo-3-chloropropane

for isolation of RNA

• Sinônimo(s): 1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
• Fórmula linear: Cl(CH₂)₃Br
• Número CAS: 109-70-6
• Peso molecular: 157.44
• Beilstein: 605278
• Número CE: 203-697-1
• Número MDL: MFCD00000998
• Código UNSPSC: 41105515
• ID de substância PubChem: 24892149
• NACRES: NA.52

Propriedades

• grau: Molecular Biology; for molecular biology
• Ensaio: ≥98.5% (GC)
• Formulário: liquid
• índice de refração: n20/D 1.486 (lit.)
• p.e.: 144-145 °C (lit.)
• densidade: 1.592 g/mL at 25 °C (lit.)
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: ClCCCBr
• InChI: 1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
• chave InChI: MFESCIUQSIBMSM-UHFFFAOYSA-N

Descrição

Descrição geral

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA.[1] It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Aplicação

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

• feline teeth[2]
• mice brain and liver tissues[3]
• human ectocervical epithelial cells[4]
• placental samples[4]
• human mesenchymal stromal cells (hMSCs)[5]

Características e benefícios

• less toxic alternative to chloroform
• compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Outras notas

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

Informações de segurança

• Pictogramas: GHS06,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H302,H331,H335,H341,H412
• Declarações de precaução: P202 - P261 - P273 - P301 + P312 - P304 + P340 + P311 - P308 + P313
• Classificações de perigo: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 2
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter