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A9207

Sigma-Aldrich

Avidin−Agarose from egg white

aqueous glycerol suspension

Sinônimo(s):

Avidin agarose

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R$ 1.086,00
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Previsão de entrega em29 de março de 2025

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1 ML
R$ 1.086,00
5 ML
R$ 3.739,00

About This Item

Número MDL:
MFCD00130572
Código UNSPSC:
41106514
NACRES:
NA.56

R$ 1.086,00

Previsão de entrega em29 de março de 2025

fonte biológica

egg white

Nível de qualidade

200

conjugado

agarose conjugate

Formulário

aqueous glycerol suspension

Matriz

cross-linked 6% beaded agarose

ativação da matriz

epichlorohydrin

ligação da matriz

amino

espaçador de matriz

15 atoms

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.[1]

Aplicação

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
  • in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )[2]
  • in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni[3]
  • in avidin bead assay for purification of labelled biotin[4]
  • for capture of glycoproteins from murine cornea samples[5]

Ações bioquímicas/fisiológicas

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications.[1] In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.[6]

Definição da unidade

One unit will bind 1.0 μg of d-biotin.

forma física

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000322714
0000255119
0000292850
0000247147
SLCH2002

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Protein A–Agarose macrobeads aqueous suspension

Millipore

P1925

Protein A–Agarose macrobeads

Streptavidin Agarose Column or batch isolation of biotinylated molecules

Millipore

69203

Streptavidin Agarose

Azide agarose

Sigma-Aldrich

900957

Azide agarose

Protein G–Agarose lyophilized powder, Contains lactose stabilizers that must be removed prior to use.

Millipore

P7700

Protein G–Agarose

Estreptavidina essentially salt-free, lyophilized powder, ≥13 units/mg protein

Sigma-Aldrich

S4762

Estreptavidina

Estreptavidina recombinant, expressed in E. coli, lyophilized powder

Sigma-Aldrich

S0677

Estreptavidina

Streptavidin

Sigma-Aldrich

189730

Streptavidin

(+)-Biotin N-hydroxysuccinimide ester ≥98% (HPLC), powder

Sigma-Aldrich

H1759

(+)-Biotin N-hydroxysuccinimide ester

Covalent modification of cytoskeletal proteins in neuronal cells by tryptamine-4, 5-dione
Kato Y, et al.
Redox Biology, 2, 983-990 (2014)
Jens Spross et al.
Analytical and bioanalytical chemistry, 402(7), 2395-2405 (2012-01-21)
A poly(glycidyl methacrylate-co-acrylamide-co-ethylene dimethacrylate) monolith and a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique. The biotin binding capacity of monolithic affinity columns with immobilized monomeric
Systematic evaluation of avidin-biotin interaction by fluorescence spectrophotometry
Sun, S., et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 89C, 99-104 (2011)
Amber L Jolly et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(6), 2393-2404 (2017-02-23)
Cell surface glycosylation is thought to be involved in barrier function against microbes at mucosal surfaces. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to
Dielectric spectroscopy of molecular interactions based on the avidin-biotin complex
Mellor, B.L., et al.
Conference proceedings : Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 3660-3663 (2011)
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