92544
Abberior® FLIP 565, maleimide
for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
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About This Item
Código UNSPSC:
12352111
NACRES:
NA.32
Produtos recomendados
forma
solid
concentração
≥50.0% (degree of coupling)
solubilidade
DMF: 0.25 mg/mL, clear
fluorescência
λex 565 nm; λem 580 nm±5 nm in PBS, pH 7.4
temperatura de armazenamento
−20°C
Descrição geral
Absorption Maximum (off-state) λmax:314 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)
Aplicação
Abberior® FLIP 565 conjugated with secondary antibody has been used for STORM (stochastic optical reconstruction microscopy) imaging of COS-7 and S180 cells.
Adequação
Designed and tested for fluorescent super-resolution microscopy
Outras notas
Informações legais
abberior is a registered trademark of Abberior GmbH
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Remi Galland et al.
Nature methods, 12(7), 641-644 (2015-05-12)
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
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