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Merck
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Documentos

66541081

Sigma-Aldrich

STBCi255-A

Human iPS Cell Line

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About This Item

Código UNSPSC:
41106509

fonte biológica

human dermis (fibroblast)

método de reprogramação

Sendai virus

descrição

age (N/A)

fabricante/nome comercial

EBiSC

sexo

female

modo de crescimento

adherent (pluripotent)

técnica(s)

cell culture | stem cell: suitable

doença(s) relevante(s)

migraine disorder

Condições de expedição

dry ice

temperatura de armazenamento

−196°C

Descrição geral

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Origem de linhagem celular

Depositor
StemBANCC

Descrição de linhagem celular

Derivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative

Genotyping
Karyotyping
Cell line karyotype: No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array

Ligação

Note: EAUA and CLIP must be completed before order fulfillment

Rotina de subcultura

Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1

Informações legais

EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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