66540624

UKKi027-C

Human iPS Cell Line

• Código UNSPSC: 41106509

Propriedades

• fonte biológica: human peripheral blood
• método de reprogramação: Sendai virus
• descrição: age (25-29)
• fabricante/nome comercial: EBiSC^™
• sexo: female
• modo de crescimento: adherent (pluripotent)
• técnica(s): cell culture | stem cell: suitable
• doença(s) relevante(s): none
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Origem de linhagem celular

Depositor
Klinikum der Universität zu Köln

Descrição de linhagem celular

Derivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
FOXD3 Yes -
CD30 Yes -
DNMT3b Yes -
TRA 1-80 Yes - -
SOX2 Yes -
NANOG Yes - -
OCT 4 Yes - -
SSEA-4 Yes - -
SSEA-1 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 17
Cell line karyotype: no larger chromosomal aberrations to be reported
Image / Result

Ligação

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Rotina de subcultura

Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 20%
CO₂ concentration: 5%
Culture medium: other
Culture base medium: StemFlex^™
Main protein source: -
Passage Number banked (pre-EBiSC): 22
No. Vials banked (pre-EBiSC): 25

Informações legais

EBiSC is a trademark of Fraunhofer-Gesellschaft

StemFlex is a trademark of Life Technologies Corporation

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable