52944
Anti-Rabbit IgG−Abberior® STAR 488 antibody produced in goat
for STED application
Sinônimo(s):
Abberior® STAR 488-Anti-Rabbit IgG antibody produced in goat
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
fonte biológica
goat
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
secondary antibodies
clone
polyclonal
forma
buffered aqueous solution
reatividade de espécies
rabbit
disponibilidade
available only in USA and Canada
concentração
~1 mg/mL
fluorescência
λex 503 nm; λem 524 nm in PBS, pH 7.4
temperatura de armazenamento
−20°C
Descrição geral
Abberior STAR 488 was developed for STED and confocal microscopy in the green spectral region. It is a bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with the prominent 488 nm laser line. For STED microscopy, Abberior STAR 488 can be efficiently used with STED Laser wavelengths between 590 - 610 nm. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto™ 488 or Alexa Fluor™ 488. Abberior STAR 488 is the ideal partner for Abberior STAR 440SXP to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Aplicação
Anti-Rabbit IgG-Abberior® STAR 488 antibody has been used as a secondary antibody:
- for fluorescent immunohistochemistry in prostate cancer cell lines, PPC-1 and TSU-Pr1
- for immunofluorescence in HeLa and monkey kidney COS-7 cells
- for STED (stimulated emission depletion) microscopy in OLN-t40-α-syn cells (oligodendroglial cells)
Adequação
Designed and tested for fluorescent super-resolution microscopy
Nota de análise
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Outras notas
Informações legais
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
produto relacionado
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Katharina Pukaß et al.
Frontiers in cellular neuroscience, 9, 163-163 (2015-05-23)
α-Synuclein (α-syn) positive glial cytoplasmic inclusions (GCI) originating in oligodendrocytes (ODC) are a characteristic hallmark in multiple system atrophy (MSA). Their occurrence may be linked to a failure of the ubiquitin proteasome system (UPS) or the autophagic pathway. For proteasomal
Giuseppe Vicidomini et al.
Methods (San Diego, Calif.), 66(2), 124-130 (2013-07-03)
Stimulation emission depletion (STED) microscopy breaks the spatial resolution limit of conventional light microscopy while retaining its major advantages, such as working under physiological conditions. These properties make STED microscopy a perfect tool for investigating dynamic sub-cellular processes in living
Combination of axitinib and dasatinib for anti-cancer activities in two prostate cancer cell lines.
Peng N
Bangladesh Journal of Pharmacology, 11, 10-10 (2016)
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica