3447-020-01

Cultrex 3-D Culture Matrix^™ Rat Collagen I

• Código UNSPSC: 12352200

Propriedades

• fonte biológica: rat
• esterilidade: sterile
• peso molecular: 300 kDa
• concentração: 5 mg/mL
• técnica(s): cell culture | mammalian: suitable
• solubilidade: water: miscible
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

The 3-D Culture Matrix^™ Rat Collagen I may be used as a gel on which to grow cells or a medium additive, alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. Type I Collagen is the major structural component of extracellular matrices found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. The 5 mg/ml concentration is very viscous for researchers wanting a thicker Collagen.

forma física

Solution in 20 mM Acetic Acid, pH 3.4 - 3.6

Outras notas

Functional Assay: 3-D Culture: Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH and promotes attachment and growth of murine endothelial SVEC4-10 cells. Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.

Sterility Testing: No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.

Storage Conditions and product stability: Product is stable for a minimum of 3 months from date of shipment if stored at 4°C. Do Not Freeze.

Informações legais

3-D Culture Matrix is a trademark of Trevigen, Inc.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable