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77730

Millipore

Gram Staining Kit

for microscopy, used for differentiation of bacteria on the basis of their gram nature.

Sinônimo(s):

Bacteria Staining Kit according to Gram

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About This Item

Código UNSPSC:
41171621
NACRES:
NA.85

grau

for microscopy

Nível de qualidade

100

Agency

according to GB 4789.30-2016

linha de produto

BioChemika

prazo de validade

limited shelf life, expiry date on the label

técnica(s)

microbe id | staining: suitable

espectro de atividade do antibiótico

Gram-negative bacteria
Gram-positive bacteria

aplicação(ões)

clinical testing
environmental
food and beverages
pharmaceutical

adequação

bacteria

Descrição geral

The Gram staining kit is used in differential staining technique that is most widely applied in all microbiology disciplines laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. There are many physiological differences between gram-positive and gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. In practice, a thin smear of bacterial cells is stained with crystal violet, then treated with iodine containing mordant to increase the binding of the primary stain. A decolorizing solution of alcohol or acetone is used to remove the crystal violet from cells which bind it weakly and then the counterstain safranin is used to provide color contrast in those cells that are decolorized. In a properly stained smear by gram staining procedure, the gram-positive bacteria appear blue to purple and gram-negative cells appear pink to red

Aplicação

The Gram staining method is one of the most important staining techniques in microbiology. It is also the most important criteria in any identification scheme for all types of bacterial isolates to differentiate the gram-positive and gram-negative bacteria.

Componentes

Kit contains (250ml each):
94448 Gram′s crystal violet Solution
90107 Gram′s iodine Solution
75482 Gram′s Decolorizer Solution
94635 Gram′s safranin Solution

Palavra indicadora

Danger

Frases de perigo

H225,H319,H336,H351,H412

Classificações de perigo

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Órgãos-alvo

Central nervous system

Perigos de suplementos

EUH066

Código de classe de armazenamento

3 - Flammable liquids

Ponto de fulgor (°F)

68.0 °F

Ponto de fulgor (°C)

20 °C

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificados de análise (COA)

Lot/Batch Number
BCCM2797
BCCM0267
BCCL8176
BCCL6405
BCCL5052

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Gram′s safranine solution for the Gram staining method

Sigma-Aldrich

1.09217

Gram′s safranine solution

Neisser’s Methylene Blue Solution for microscopy

Sigma-Aldrich

01363

Neisser′s Methylene Blue Solution

Gram Stain TISSUE-TROL™ Control Slides mouse lung tissue containing Staphylococcus aureus and Escherichia coli

Sigma-Aldrich

TTR005

Gram Stain TISSUE-TROL™ Control Slides

Prevalence of blaOXA-?-Lactamase-Producing and Methicillin-Resistant Staphylococcus aureus in Iran
Navidinia M
AANA Journal (2016)
Antimicrobial efficacy of fermented dark vinegar from unpolished rice
Cho HJ
Microbiology and Biotechnology Letters (2015)
U E Rodríguez-Castrejón et al.
Archives of microbiology, 204(3), 191-191 (2022-02-24)
The study of arsenic (As)-resistant microorganisms with high As removal capacity is fundamental for the development of economically sustainable technologies used for the treatment of water contaminated with metalloid. In the current study, four bacterial strains were isolated from As-contaminated
Hiral Mistry et al.
PloS one, 11(9), e0162256-e0162256 (2016-09-08)
Bovine mastitis caused by multidrug resistant Staphylococcus aureus is a huge problem reported worldwide, resulting in prolonged antibiotic treatment and death of livestock. The current study is focused on surveillance of antibiotic susceptibility along with genotypic and phenotypic characterization of
Potential Threapeutic Applications of Extract Made from Electroporated Xenopus
Laevis frog Oocytes in Murine Models of Melanoma, Traumatic Brain Injury and
Experimental Skin Wrinkling
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Bjog : An International Journal of Obstetrics And Gynaecology (2016)
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