00879

8-Acetoxypyrene-1,3,6-trisulfonic acid trisodium salt

for fluorescence, ≥98.0% (TLC)

• Sinônimo(s): 1-Acetoxypyrene-3,6,8-trisulfonic acid trisodium salt, Trisodium-8-acetoxypyrene-1,3,6-trisulfonate
• Fórmula empírica (Notação de Hill): C₁₈H₉Na₃O₁₁S₃
• Número CAS: 115787-83-2
• Peso molecular: 566.42
• Beilstein: 9538267
• Número MDL: MFCD00135685
• Código UNSPSC: 12352200
• ID de substância PubChem: 24845099

Propriedades

• grau: for fluorescence
• Ensaio: ≥98.0% (TLC)
• pf: 238-248 °C (dec.) (lit.)
• solubilidade: DMF: soluble; DMSO: soluble; H₂O: soluble; methanol: soluble
• fluorescência: λ_ex 460 nm; λ_em 510 nm in 0.1 M potassium phosphate pH 8.0 (esterase)
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: [Na+].[Na+].[Na+].CC(=O)Oc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

Descrição

Aplicação

A membrane permeable, nonfluorescent reagent . This fluorochrome enters the cell cytoplasm, where its acetic group is cleaved by intracellular, nonspecific esterases, which forms the impermeable, fluorescent form of the molecule. Utilized as a phloem-mobile symplasmic tracer having staining characteristics that differ from the commonly used CFDA (5-(6-)carboxyfluorescein diacetate).

suitable as fluorogenic substrate for esterases

Outras notas

Highly water soluble enzyme substrate for the fluorimetric assay of esterases at longwave excitation and emission wavelengths