11371517001

Swa I

from Staphylococcus warneri

• Código UNSPSC: 12352204

Propriedades

• Formulário: solution
• embalagem: pkg of 200 U (10 U/μl)
• fabricante/nome comercial: Roche
• Parâmetros: 25 °C optimum reaction temp.
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Especificidade

Recognition sites: ATTTAAAT
ATTTAAAT
Restriction site: ATTT↓AAAT
ATTT↓AAAT
Heat inactivation: Swa I can be heat inactivated by incubation at 65 °C for 20 minutes.

Aplicação

Swa I is very useful for digesting GC-rich bacterial genomes.

Perfil do DNA

Number of cleavage sites on different DNAs

• λ: 0
• φX174: 0
• Ad2: 1
• M13mp7: 1
• pBR322: 0
• pBR328: 0
• SV40: 1

Definição da unidade

One unit is the enzyme activity that completely cleaves 1 μg Ad2 × Ssp I DNA in one hour at +25 °C in a total volume of 25 μl (1x) SuRE/Cut buffer H.

Nota de análise

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

• A: 0-10%
• B: 10-25%
• H: 100%
• L: 0-10%
• M: 0-10%

Activity in PCR buffer: Not tested

Outras notas

For life science research only. Not for use in diagnostic procedures.

Somente componentes do kit

• Enzyme Solution
• SuRE/Cut Buffer H 10x concentrated

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash