ST1049

Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

liquid, clone MCP231, Calbiochem^®

• Código UNSPSC: 12352203

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: MCP231, monoclonal
• Formulário: liquid
• contém: ≤0.1% sodium azide as preservative
• reatividade de espécies: human, yeast, rat, rabbit, mouse
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG1
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PSMA1(5682)

Descrição

Descrição geral

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.

Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.

This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits.

Imunogênio

Epitope: within the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)

dinitrophenylated proteasomes

Aplicação

Immunoblotting (1:1000, chemiluminescence)
Immunoprecipitation (not recommended)
ELISA (see comments)
Immunofluorescence (see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Frozen Sections (see application references)

Advertência

Toxicity: Highly Toxic (H)

Nota de análise

Positive Control
Human placental tissue

Outras notas

Immunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.

Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA94, 2939.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable