PC635

PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

liquid, Calbiochem^®

• Sinônimo(s): Anti-Nibrin (pSer³⁴³) Rabbit pAb
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: diluted serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: liquid
• não contém: preservative
• reatividade de espécies: human
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: phosphorylation (pSer343)
• Informações sobre genes: human ... NBN(4683)

Descrição

Descrição geral

Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser³⁴³.

Recognizes the ~95 kDa nibrin protein phosphorylated at Ser³⁴³ in δ-irradiated lymphoblastoid cells.

This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

Imunogênio

Human

a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid

Aplicação

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 µl/500 µg cell extract)

Advertência

Toxicity: Standard Handling (A)

forma física

Serum diluted in PBS.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Nota de análise

Positive Control
γ-irradiated lymphoblastoid cells

Outras notas

Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.

This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable