MABT855M

Anti-Podoplanin Antibody, clone LpMab-13

clone LpMab-13, from mouse

• Sinônimo(s): Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: LpMab-13, monoclonal
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μg
• técnica(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_006465.3
• nº de adesão UniProt: Q86YL7
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PDPN(10630)

Descrição

Descrição geral

Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-13 binds to PLAG2/3 domains and recognizes endogenous hPDPN of cancer cells, including glioblastoma, oral cancer, lung cancer, and malignant mesothelioma, and normal cells such as lymphatic endothelial cells and podocytes of kidney. The interaction between LpMab-13 and hPDPN is independent of its glycosylation. The minimum epitope of LpMab-13 is identified to be Ala42-Asp49 of hPDPN. ((Ref.: Ogasawara, S., et al. (2016). Monoclon. Antib. immunodiag. Immunother. 35(3); 155-163).

Especificidade

Clone LpMab-13 detects Podoplanin in human cells. The minimal epitope is shown to be Ala42-Asp49.

Imunogênio

LN229 glioma cells expressing human Podoplanin.

Aplicação

Anti-Podoplanin, clone LpMab-13, Cat. No. MABT855, is a mouse monoclonal antibody that detects human podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

ELISA Analysis: A representative lot detected Podoplanin in ELISA application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(3):155-62).

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(3):155-62).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(3):155-62).

Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(3):155-62).

Research Category
Cell Structure

Qualidade

Evaluated by Immunohistochemistry in human testis cancer tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human testis cancer tissue.

Descrição-alvo

16.70 kda calculated.

forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable