About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
conjugado:
unconjugated
application:
WB
clone:
3D2 also termed M24N 3D2, monoclonal
reatividade de espécies:
mouse, human
citations:
técnica(s):
western blot: suitable
fonte biológica
rat
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
3D2 also termed M24N 3D2, monoclonal
peso molecular
calculated mol wt 70.46 kDa
reatividade de espécies
mouse, human
embalagem
antibody small pack of 100 μg
técnica(s)
western blot: suitable
Isotipo
IgG2aκ
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
2-8°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
mouse ... Mmp24(17391)
Descrição geral
Matrix metalloproteinase-24 (UniProt: Q9R0S2; also known as MMP-24, Matrix metalloproteinase-21, MMP-21, Membrane-type matrix metalloproteinase 5, MT-MMP 5, MTMMP5, Membrane-type-5 matrix metalloproteinase, MT5-MMP, MT5MMP) is encoded by the Mmp24 (also known as Mmp21, Mt5mmp) gene (Gene ID: 17391) in murine species. MMP-24 is a single-pass type I membrane protein that is mainly expressed in neuronal cells of both central and peripheral nervous systems. It mediates the cleavage of N-cadherin and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. It is also involved in cell-cell interactions between nociceptive neurites and mast cells. MMP-24 is synthesized with a signal peptide (aa 1-41) and a propeptide (aa 42-128) that are subsequently cleaved off to generate functional MMP-24 (aa 129-618) or processed MMP-24 (aa 129-554). MMP-24 contains a cysteine switch motif (aa 110-117) that binds the catalytic zinc ion and inhibits its enzyme activity. The dissociation of the cysteine from the zinc ion upon the activation-peptide release results in the activation of enzyme.
Especificidade
Clone 3D2 is a rat monoclonal antibody that detects MT5-MMP/MMP-24. It targets an epitope within 16 amino acids from the extracellular domain.
Imunogênio
Ovalbumin-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human MT5-MMP.
Aplicação
Quality Control Testing
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
Tested Applications
Western Blotting Analysis: A representative lot detected MT5-MMP/MMP24 in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).
Western Blotting Analysis: A 1:200 dilution from a representative lot detected MT5-MMP/MMP24 in lysate from HEK293 cells over expressing MT5-MMP, but not in HEK293 cells over expressing MT1-MMP and in mock control HEK293 cell lysates (Negative). (Courtesy of Michael Willem, Biomedical Center, Ludwig Maximilian University of Munich, Germany).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
Tested Applications
Western Blotting Analysis: A representative lot detected MT5-MMP/MMP24 in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).
Western Blotting Analysis: A 1:200 dilution from a representative lot detected MT5-MMP/MMP24 in lysate from HEK293 cells over expressing MT5-MMP, but not in HEK293 cells over expressing MT1-MMP and in mock control HEK293 cell lysates (Negative). (Courtesy of Michael Willem, Biomedical Center, Ludwig Maximilian University of Munich, Germany).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MT5-MMP/MMP24, clone 3D2, Cat. No. MABT1549, is a rat monoclonal antibody that detects MT5-MMP and is tested for use in Western Blotting.
forma física
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at +2°C to +8°C from date of receipt.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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