MABS1720

Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2

clone 6C1.2, from mouse

• Sinônimo(s): Acetyl-coenzyme A synthetase, cytoplasmic, Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propriedades

• fonte biológica: mouse
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 6C1.2, monoclonal
• reatividade de espécies: human
• técnica(s): western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_061147
• nº de adesão UniProt: Q9NR19
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ACSS2(55902)

Descrição

Descrição geral

Acetyl-coenzyme A synthetase, cytoplasmic (EC 6.2.1.1; UniProt Q9NR19; also known as Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2) is encoded by the ACSS2 (also known as ACAS2) gene (Gene ID 55902) in human. ACS plays a vital role in prokaryotic and eukaryotic cells by activating acetate to form acetyl CoA that serves as a source of two carbon metabolite for lipid synthesis and energy producing reactions. It is also reported to play a role in gene expression. In yeast cells it delivers acetyl CoA for histone acetylation. ACS contributes to cancer cell growth under low-oxygen and lipid-depleted conditions and its expression is upregulated under metabolically stress. Silencing ACSS2 gene is shown to reduce the growth of tumor xenografts. Ref.: Starai, VJ., and Escalante-Semerena, JC. (2004). Cell. Mol. Life Sci. 61, 2020 2030. Schug, ZT et al. (2015). Cancer Cell. 27:57-71.

Especificidade

Immunogen sequence is present in both spliced isoforms of human acetyl-CoA synthetase reported by UniProt (Q9NR19).

Imunogênio

GST-tagged recombinant human acetyl-CoA synthetase N-terminal fragment.

Aplicação

Detect Acetyl-CoA synthetase using this mouse monoclonal Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2, Cat. No. MABS1720, validated for use in Western Blotting.

Qualidade

Evaluated by Western Blotting in HEPG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl-CoA synthetase in 10 µg of HepG2 cell lysate.

Descrição-alvo

~80 kDa observed. 78.58/80.09 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable