Pular para o conteúdo
Merck
Todas as fotos(3)

Key Documents

MABN2614

Sigma-Aldrich

Anti-LOTUS Antibody, clone H24G11

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
NACRES:
NA.74

fonte biológica

hamster

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

H24G11, monoclonal

peso molecular

calculated mol wt 70.33 kDa
observed mol wt ~85 kDa

purificado por

using protein G

reatividade de espécies

mouse

embalagem

antibody small pack of 100 μL

técnica(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotipo

IgGκ

sequência de epítopo

Unknown

nº de adesão de ID de proteína

nº de adesão UniProt

Condições de expedição

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

mouse ... Crtac1(72832)

Descrição geral

Cartilage acidic protein 1 (UniProt: Q8R555; also known as 68 kDa chondrocyte-expressed protein, CEP-68, ASPIC, Protein CRTAC1-B, Lateral olfactory tract usher substance, LOTUS) is encoded by the Crtac1 (also known as Aspic1, Cep68) gene (Gene ID: 72832) in murine species. LOTUS is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form (aa 29-646). It is a membrane-bound and/or secreted protein that serves as an endogenous Nogo receptor-1 (NgR1) antagonist. It is shown to contribute to the formation of the lateral olfactory tract (LOT) axonal bundle. Endogenous Nogo-A signaling through NgR1 is shown to induce axonal branching. Developmental up-regulation of Nogo-A promotes collateral formation of the LOT during the late development period. It has been reported that axon growth inhibitors, which include Nogo proteins, myelin-associated glycoprotein (MAG), oligodendrocyte myelin glycoprotein (OMgp), and B lymphocyte stimulator (BLyS), bind to NgR1 and restrict functional recovery after damage to the adult central nervous system. LOTUS blocks the binding of these ligands to NgR1 and suppresses growth cone collapse. LOTUS knockout mice show significantly increased collateral branches of axons in the LOT. On the other hand, this branching is diminished in NgR1 knockout mice. A simultaneous loss of LOTUS and NgR1 is reported to rescue the abnormal increase in axonal branches. (Ref.: Iketani, M., et al. (2016). Sci. Rep. 6; 39586; Kurihara, Y., et al. (2014). Mol. Cell. Neurosci. 61; 211-218).

Especificidade

Clone H24G11 is a hamster monoclonal antibody that detects murine cartilage acidic protein 1B (LOTUS).

Imunogênio

Homogenate of protein extract from developing lateral olfactory tract and the surrounding tissues of mouse.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in Mouse E14 brain tissue lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LOTUS in Mouse E14 brain tissue lysates.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected LOTUS in Immunocytochemistry applications (Kurihara, Y., et al. (2014). Mol Cell Neurosci. 61:211-8; Sato, Y., et al. (2011). Science. 333(6043):769-73).

Immunohistochemistry Applications: A 1:50 dilutions from a representative lot detected LOTUS in E14 embryonic mouse tissue sections.

Western Blotting Analysis: A representative lot detected LOTUS in Western Blotting applications (Iketani, M., et al. (2016). Sci Rep. 6:39586).

Immunohistochemistry Applications: A representative lot detected LOTUS in Immunohistochemistry applications ( Sato, Y., et al. (2011). Science. 333(6043):769-73; Iketani, M., et al. (2016). Sci Rep. 6:39586).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LOTUS, clone H24G11, Cat. No. MABN2614, is a hamster monoclonal antibody that detects Cartilage acidic protein 1 (LOTUS) and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

forma física

Purified hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica