MABF2299

Anti-IL-1α Antibody, clone 7E5.14.5

• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 7E5.14.5, monoclonal
• peso molecular: calculated mol wt 30.61 kDa; observed mol wt ~18 kDa
• purificado por: using protein G
• reatividade de espécies: human
• embalagem: antibody small pack of 100 μg
• técnica(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG1κ
• sequência de epítopo: Unknown
• nº de adesão de ID de proteína: NP_000566.3
• nº de adesão UniProt: P01583
• Condições de expedição: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Interleukin-1 alpha (UniProt: P01583; also known as IL-1 alpha, Hematopoietin-1) is encoded by the IL1A (also known as IL1F1) gene (Gene ID: 3552) in human. IL-1a is constitutively expressed in several healthy tissues in a steady state and its expression can be increased in response to a number of stimuli. Absolute amounts of IL-1a vary among cell types, but barrier cells, such as endothelial and epithelial cells, express higher amounts at steady state. IL-1a is synthesized with a propeptide (aa 1-112) and is post-translationally modified by phosphorylation at serine 90, myristoylation on lysine 82, and acetylation on lysine 82. After synthesis, pro-IL-1a is localized into the nucleus, cytosol or lysosomal compartment or is displayed on the outer leaflet of the plasma membrane. Its intracellular localization can change in response to specific stimuli. Pro-IL-1a can also be cleaved by calpain to generate IL-1 -N-terminal domain (NTP) and a mature C-terminal IL-1a form. The C-terminal domain is catalytically active and is capable of signaling via IL-1R. Both IL-1a -NTP and pro-IL-1a contains a functional NLS signal and bind HAX1 to allow translocation into the nucleus. In the nucleus, IL-1a -NTP binds transcription regulation factors and activates expression of proinflammatory cytokines and chemokines independently of IL-1R1 signaling. The biological functions of IL-1a also involve stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. (Ref.: Malik, A., and Kanneganti, TD. (2018). Immunol. Rev. 281(1); 124-137; Di Paolo, NC., and Shayakhmetov, DM (2016). Nat. Commun. 17(8); 906-913).

Especificidade

Clone 7E5.14.5 is a mouse monoclonal antibody that detects human IL-1a .

Imunogênio

Full-length recombinant human IL-1alpha (without the propeptide).

Aplicação

Quality Control Testing

Evaluated by Western Blotting with human recombinant IL-1a.

Western Blotting Analysis: A 1,000 dilution of this antibody detected human recombinant IL-1a.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1a in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-1a, clone 7E5.14.5, Cat. No. MABF2299, is a mouse monoclonal antibody that detects Interluekin-1a and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

forma física

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Armazenamento e estabilidade

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable