MABE867
Anti-Mad1 Antibody, clone BB3-8
clone BB3-8, from mouse
Sinônimo(s):
Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, Mitotic checkpoint MAD1 protein homolog, HsMAD1, hMAD1, Tax-binding protein 181
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
BB3-8, monoclonal
reatividade de espécies
human
técnica(s)
immunofluorescence: suitable
western blot: suitable
Isotipo
IgG1κ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... MAD1L1(8379)
Categorias relacionadas
Descrição geral
Mitotic arrest deficient-like 1 (yeast) is also known as MAD1 and MAD1L1. It is part of the MAD1 family which is crucial to development. MAD1 acts as a checkpoint during mitotic spindle assembly. During anaphase and telophase MAD1 is located at the spindle mid-zone of the cell, where it prevents anaphase until the chromosome is aligned at the metaphase plate. During metaphase MAD1 is located at the centrosome. MAD1 participates in cell cycle control and tumor suppression. MAD1 functions as a homodimer and interacts with MAD2L1. It is thought that MAD1 recruits to MAD2, which then promotes binding of MAD2 to CDC20.
Imunogênio
His-tagged recombinant protein corresponding to human Mad1.
Aplicação
Anti-Mad1 Antibody, clone BB3-8 is a highly specific mouse monoclonal antibody, that targets MAD1 & has been tested in western blotting & Immunofluorescence.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Qualidade
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.
Descrição-alvo
~83 kDa observed
forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Andrea Corno et al.
The EMBO journal, 42(20), e112630-e112630 (2023-09-15)
Two major mechanisms safeguard genome stability during mitosis: the mitotic checkpoint delays mitosis until all chromosomes have attached to microtubules, and the kinetochore-microtubule error-correction pathway keeps this attachment process free from errors. We demonstrate here that the optimal strength and
Jingchao Wu et al.
The Journal of cell biology, 223(1) (2023-11-07)
Correct chromosome segregation during cell division depends on proper connections between spindle microtubules and kinetochores. During prometaphase, kinetochores are temporarily covered with a dense protein meshwork known as the fibrous corona. Formed by oligomerization of ROD/ZW10/ZWILCH-SPINDLY (RZZ-S) complexes, the fibrous
Shawn Yost et al.
Nature genetics, 49(7), 1148-1151 (2017-05-30)
Through exome sequencing, we identified six individuals with biallelic loss-of-function mutations in TRIP13. All six developed Wilms tumor. Constitutional mosaic aneuploidies, microcephaly, developmental delay and seizures, which are features of mosaic variegated aneuploidy (MVA) syndrome, were more variably present. Through
Ana Margarida Gomes et al.
Current biology : CB, 32(19), 4240-4254 (2022-09-04)
Chromosome alignment to the spindle equator is a hallmark of mitosis thought to promote chromosome segregation fidelity in metazoans. Yet chromosome alignment is only indirectly supervised by the spindle assembly checkpoint (SAC) as a byproduct of chromosome bi-orientation, and the
Chu Chen et al.
Current biology : CB, 29(1), 104-119 (2019-01-01)
Switch-like activation of the spindle assembly checkpoint (SAC) is critical for accurate chromosome segregation and for cell division in a timely manner. To determine the mechanisms that achieve this, we engineered an ectopic, kinetochore-independent SAC activator: the "eSAC." The eSAC
Active Filters
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
