MABE429

Anti-UBE2I Antibody, clone 1B10

ascites fluid, clone 1B10, from mouse

• Sinônimo(s): SUMO-conjugating enzyme UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: 1B10, monoclonal
• reatividade de espécies: monkey, human
• técnica(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1
• nº de adesão UniProt: P63279
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... UBE2I(7329)

Descrição

Descrição geral

The protein encoded by the human gene UBE2I (or UBC9, UBCE9) is a SUMO conjugating enzyme, also called SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I or p18, that attaches SUMO proteins (SUMO1, SUMO2, SUMO3 & SUMO4) to other target proteins, primarily various transcription factors (HIF1A, FOXL2, KAT5, I-kappa-B) with the help of ubiquitin E3 ligases such as CBX4 and RANBP2. UBE2I is also know to interact with a huge array of additional targets as part of its role as a ubiquitin E2 ligase including SALL1, FHIT, RANBP2, Protein inhibitor of activated STAT2, DNMT3A, DNMT3B, Small ubiquitin-related modifier 1, SAE2,RANGAP1,Death associated protein 6, Androgen receptor, ETS1, IPO13, TCF3, TNFRSF1A, DACH1, P53, BLMH, MAP3K1, WT1, TOP1, RAD51, Activating transcription factor 2, and PIAS1. UBE2I expression is largely nuclear and the protein can be found expressed in a wide variety of tissues including heart, skeletal muscle, pancreas, kidney, liver, lung, testis, thymus, placenta and brain. EMD-Millipore’s Anti-UBE2I mouse monoclonal antibody has been tested in western blot on recombinant UBE2I protein as well as HeLa, HepG2 and COS7 cell lysates and in paraffin embedded immunohistochemistry on normal prostate and bladder cancer tissue sections. The antibody has also been successfully tested in fluorescent immunocytochemistry on HepG2 cells in culture and in flow cytometry on HepG2 cells in culture.

Imunogênio

Purified recombinant fragment of human UBE2I expressed in E. Coli.

Aplicação

Anti-UBE2I Antibody, clone 1B10 is a highly specific mouse monoclonal antibody, that targets Ubiquitin carrier protein 9 & has been tested in western blotting, Immunofluorescence, IHC & Flow Cytometry.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected UBE2I in prostate and bladder cancer tissues.

Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected UBE2I in HepG2 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected UBE2I in non serum starved HepG2 cells.

Optimal working dilutions must be determined by end user.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualidade

Evaluated by Western Blotting in HeLa, HepG2, and Cos7 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected UBE2I in HeLa, HepG2, and Cos7 cell lysates.

Descrição-alvo

~18 kDa observed. Uncharacterized bands may appear in some lysate(s).

forma física

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
HeLa, HepG2, and Cos7 cell lysates

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable