MABE263

Anti- Histone H3c1 Antibody

mouse monoclonal, 9B1-2G6

• Sinônimo(s): Histone H3.1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• Nome do produto: Anti-unmethylated-Histone H3 (Lys9) Antibody, clone 9B1-2G6, clone 9B1-2G6, from mouse
• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 9B1-2G6, monoclonal
• reatividade de espécies: human
• técnica(s): dot blot: suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_038578
• nº de adesão UniProt: P68433
• Condições de expedição: wet ice
• Informações sobre genes: human ... H3C1(8350)

Descrição

Descrição geral

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Conversely,deacetylation of histone H3 lysine 9 is associated with gene-activating events. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.

Especificidade

This antibody recognizes Histone H3 unmethylated at Lys9. This antibody does not cross react with methyl-Histone (Lys29). This antibody detects unmethylated Histone H3 (Lys9) when Ser10 is phosphorylated. It also detects mono-/di-methyl-Histone H3 (Lys4), and Acetyl-Histone H3 (Lys14). (Prof. E. Ogris, Department of Medical Biochemistry, Medical University of Vienna).

Imunogênio

Epitope: unmethylated Lys9

KLH-conjugated linear peptide corresponding to human unmethylated Histone H3 (Lys9).

Aplicação

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-unmethylated-Histone H3 (Lys9) Antibody, clone 9B1-2G6 (Mouse Monoclonal Antibody) demonstrated to detect unmethylated-Histone H3 (Lys9) also known as Histone H3.1.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected unmethylated Histone H3 (Lys9) in 10 µg of HeLa acid extract.

Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No MABE263-S, unmethylated-Histone H3 (Lys9), clone 9B1-2G6 at 0.5 µg/mL (1:2,000) dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.

Dot Blot (Specificity) Analysis: A representative lot detected non-methylated Histone H3 (Lys9) in Dot Blot. This antibody detects non-methylated Histone H3 (Lys9) when Ser10 is phosphorylated. It also detects mono-/di-methyl-Histone H3(Lys4), and Acetyl-Histone H3 (Lys14). (Prof. E. Ogris, Department of Medical Biochemistry, Medical University of Vienna).

Qualidade

Evaluated by Western Blot in recombinant Histone H3.

Western Blot Analysis: 0.5 µg/mL of this antibody detected unmethylated Histone H3 (Lys9) in 10 µg of recombinant Histone H3.

Descrição-alvo

~15 kDa observed

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Recombinant Histone H3

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable