MABC954

Anti-MUTYH Antibody, clone 12D5.1

clone 12D5.1, from mouse

• Sinônimo(s): A/G-specific adenine DNA glycosylase, MutY homolog, hMYH
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 12D5.1, monoclonal
• reatividade de espécies: human
• técnica(s): western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_036354
• nº de adesão UniProt: Q9UIF7
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MUTYH(4595)

Descrição

Descrição geral

A/G-specific adenine DNA glycosylase (UniProt Q9UIF7; also known as hMYH, MutY homolog) is encoded by the MUTYH (also known as MYH) gene (Gene ID 4595) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. MUTYH/MYH is a monofunctional DNA glycosylase that excises mismatched adenine opposite 7, 8-dihydro-8-oxoguanine (8-oxoG), which is the major mutagenic lesion induced by oxidative stress. Biallelic mutations in MUTYH are associated with MUTYH-Associated polyposis (MAP) and increased risk in colorectal cancer (CRC).

Especificidade

Expected to react with all six spliced isoforms of human MUTYH reported by UniProt (Q9UIF7).

Imunogênio

Epitope: N-terminal half.

GST-tagged recombinant internal fragment from the N-terminal half of human MUTYH.

Aplicação

Anti-MUTYH Antibody, clone 12D5.1 is an antibody against MUTYH for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualidade

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MUTYH in 10 µg of Raji cell lysate.

Descrição-alvo

~59 kDa observed. 60.07 kDa (isoform Alpha-1), 59.14 kDa (isoform Alpha-2), 59.07 kDa (isoform Alpha-3), 58.44 kDa (isoform Beta-1), 57.52 kDa (isoform Gamma-2), and 50.52 kDa (isoform Gamma-3) calculated.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable